Areas of study include, but are not limited to:
Routes of Administration:
Mouse and Rat Protocols:
BiCellFectamaxVivo transfection of mouse liver and lung.
Left panel: molecular components of BiCellFectamaxVivo reagent.
Right panel: validation of GFP expression in the liver and the lung via tail vein injection of GFP expressing plasmid with BiCellFectamaxVivo to 6-week-old mouse.
Lentivirus production and infection of mouse embryo.
Top panel: Lentiviral backbone vector features transgenic expression cassette flanked by 5’-LTR and 3’-LTR. Middle panel: Packaged virions were used to transduce mouse zygote cells for transgenic expression, in this case, GFP expression, and examined at 2-cell, 4-cell, 8-cell and morula stage.
Bottom panel: Transduced mouse zygotes were allowed to develop to term and examined for GFP expression in a variety of tissues.
IVC Barrier Rodent Housing System
System details: Alientown EasyCage IVC mouse facility with EcoFlow airflow management system and bioBubble barrier system with HEPA filter
Barrier facility for rodent housing.
Allentown LLC has custom built a barrier facility for rodent that is capable of handling “nude mouse” as well as performing mouse T cell harvesting and manipulation. The new animal facility has triple layers of HVAC systems and HEPA filters to provide pathogen free environment for animal research.