Mouse retina labeled with NPHP5 antibody

Cryosection of C57BL/6 mouse retina was labeled with NPHP5 antibody (green). Blue: Nucleus.

Experimental condition: Mouse retina was freshly dissected and rapidly frozen with dry ice, followed by cryosectioning at –20°C. Cryosection was fixed with methanol at –20°C for 20min. Primary and secondary antibodies were incubated with the fixed section in the presence of fetal bovine serum and Tween-20.

NPHP5 antibody: BiCell Scientific Cat No: 90005.

Observation: NPHP5 proteins are found in the the primary cilia of the photoreceptor cells as well as of the interneurons in the retina.

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