Methanol fixed mIMCD3 cells were labeled with ARL13B antibody (Green). Blue: nuclei.
Experimental condition: Mouse kidney collecting duct mIMCD3 cells were fixed with methanol at –20°C for 1hr. Primary and secondary antibodies were incubated with the fixed cells in the presence of fetal bovine serum and Tween-20.
ARL13B antibody: BiCell Scientific Cat No: 90413.