Cryosection of C57BL/6 mouse embryo at E15.5 was labeled with VCAM1 antibody (green) and Desmin antibody (Red). Blue: nucleus.
Experimental condition: Mouse embryo was freshly dissected and rapidly frozen with dry ice, followed by cryosectioning at –20°C. Cryosection was fixed with methanol at –20°C for 20min. Primary and secondary antibodies were incubated with the fixed section in the presence of fetal bovine serum and Tween-20.