Cryosection of C57BL/6 mouse embryo at E15.5 was labeled with FAT1 antibody (green). Blue: nucleus. Experimental condition: Mouse embryo was freshly dissected and rapidly frozen with dry ice, followed by cryosectioning at –20°C. Cryosection was fixed with methanol at –20°C for 20min. Primary and secondary antibodies were incubated with the fixed section in the presence…
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