Cryosection of C57BL/6 mouse embryo at E15.5 was labeled with EpCAM antibody (green) and PCDH15 antibody (Red). Blue: nucleus. Experimental condition: Mouse embryo was freshly dissected and rapidly frozen with dry ice, followed by cryosectioning at –20°C. Cryosection was fixed with methanol at –20°C for 20min. Primary and secondary antibodies were incubated with the fixed…
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