Methanol fixed human RPE1 cells were labeled with Clathrin Heavy Chain (CLTC) antibody (Green). Blue: nuclei.
Experimental condition: Human retina pigmented epithelium RPE1 cells were fixed with methanol at –20°C for 1hr. Primary and secondary antibodies were incubated with the fixed cells in the presence of fetal bovine serum and Tween-20.
Clathrin Heavy Chain (CLTC) antibody: BiCell Scientific Cat No: 31060.